The by-poll for the parliamentary seat of Beed, vacated following the death of BJP leader Gopinath Munde, will also be held on October 15.
"From today, the election process has begun with filing of nominations," a Chief Electoral Office official said.
Maharashtra's over 8.28 crore voters are eligible to exercise their franchise in the single-phase to elect its 13th assembly.
September 27 is the deadline for filing nomination. After scrutiny of papers, the last date for withdrawal of nomination is October 1. Counting of votes will take place on October 19. The existing assembly's term expires on November 8.
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Electronic voting machines will be used in all the assembly constituencies. All will have a "none of the above" (NOTA) option button.
EVMs in 13 assembly constituencies in Maharashtra will also be equipped with voter verifiable paper audit trail (VVPAT).
VVPAT is a system where voters will get to verify if their votes have been cast correctly.