The order, under Prevention of Money Laundering (PMLA) laws, has been issued against the firm Mohan India Private Limted, which includes funds in bank accounts to the tune of Rs 59.53 crore and a land plot in Delhi worth Rs 2.08 crore.
The firm is one of the largest borrowers in the businesses of this exchange, ED sources said.
ED had earlier registered a criminal complaint under PMLA in this case.
An attachment action under money laundering laws is meant to deprive the accused of the benefits of the ill-gotten property or assets.
Assets worth Rs 125 crore of the company have already been seized by the agency.