The matter was reported to police two days back by the nurses, undergoing internship at the RD Gardi Medical College, who alleged that the chief medical officer and hospital superintendent asked them to get into sex trade to make money and also tried to exploit them sexually, police said.
A case was registered against the officials at Chimanganj police station here on Monday.
The complainants also alleged that they reported the matter to their matron, who also advised them to remain mum on the issue.
The other two officials against whom the complaint was filed managed to escape before police could apprehend them.
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However, denying the allegation, the college management claimed that since the trainee nurses did not want to do night shifts therefore they filed a "false complaint" against the institution.
Meanwhile, Madhya Pradesh Women's Commission member Snehlata Upadhyaya reached the college yesterday to probe the matter.
She also met college Dean Dr JK Sharma, medical superintendent Shailendra Sharma, additional Dean Dr A K Singh and nursing college in-charge Lata Sethi on the issue.
Upadhyaya has also sought a report from Ujjain's superintendent of police on the issue.