"The Classic Maya revered their divine rulers and treated them as living souls after death," said David Freidel, professor at Washington University in the US.
"This king's tomb helped to make the royal palace acropolis holy ground, a place of majesty, early in the history of the Wak - centipede - dynasty," said Freidel.
"It's like the ancient Saxon kings England buried in Old Minister, the original church underneath Winchester Cathedral," he said.
Previous research at the site has revealed six royal tombs and sacrificial offering burials dating to the fifth, sixth and seventh centuries AD.
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El Peru-Waka' is about 40 miles west of the famous Maya site of Tikal near the San Pedro Martir River in Laguna del Tigre National Park.
In the Classic period, this royal city commanded major trade routes running north to south and east to west.
One of the earliest known Maya dynasties, the Wak is thought to have been established in the second century AD based on calculations from a later historical text at the site.
Although the ruler in Burial 80, identified as a mature man, was not accompanied by inscribed artifacts and is therefore anonymous, he is possibly King Te' Chan Ahk, a historically known Wak king who was ruling in the early fourth century AD, researchers suggest.
Identification of the tomb as royal is based on the presence of a jade portrait mask depicting the ruler with the forehead hair tab of the Maize God.
Researchers discovered the mask under the head of the ruler, and it may have been made to cover the face rather than as a chest pectoral.
Archaeologists at Tikal in the 1960s discovered a similar greenstone mask in the earliest Maya royal tomb, dating to the first century AD.
Additional offerings in Burial 80 included 22 ceramic vessels, Spondylus shells, jade ornaments and a shell pendant carved as a crocodile. The remains of the ruler and some ornaments like the portrait mask were painted bright red.