Olympic Council of Asia (OCA) president Sheikh Ahmad Al-Fahad Al-Sabah said the 13,000 athletes and officials at the two week Games had to act with "goodwill and (appropriate) behaviour."
The OCA on Wednesday expelled an Iranian team official for sexually harassing a female volunteer and issued strong warnings following an allegation against a Palestinian footballer.
The Asian body called on Iran to send home equipment manager Amereh Ahmad for verbally harassing the volunteer on Monday.
The unnamed player has been banned from leaving the country. South Korean police said Friday they had finished their investigation and sent the case to prosecutors to decide if charges should be pressed.
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The organising committee has put up signs in the Games village warning against harassment, while the OCA said it had "zero tolerance" for such cases.
"I hope that over the next 16 days our athletes and you will participate with good will and (appropriate) behaviour," the Kuwaiti said ahead of the official opening ceremony.
"Enjoy your stay in Incheon, work hard for the success of the Games. Let us prepare a good environment for our movement," Sheikh Ahmad added in brief comments before media were asked to leave the room.