It will be the first interview of the cyclist since the doping controversy, said The Hollywood Reporter.
In the 90-minute interview, which will take place at Armstrong's Austin, Texas, home and air on January 17, Armstrong will address the alleged doping scandal, years of accusations of cheating and charges of lying about the use of performance-enhancing drugs throughout his storied cycling career.
Winfrey has in the past interviewed many high-profile people. She most recently spoke to pop singer Rihanna, who talked about her sometimes abusive relationship with Chris Brown.
And her March exclusive with Bobbi Kristina, the daughter of the late Whitney Houston, was another highlight of her talk show.