The memoir will use her life story as the source for inspirational advice, said The Hollywood Reporter.
"All of my experiences, even the painful ones, have been there to teach me something about life. I'm still learning and I hope my story inspires other people to live the highest, fullest expression of themselves," Winfrey said in a statement.
The 61-year-old media mogul is in the earliest stages of the writing process.
Bob Miller, president and publisher of Flatiron Books, termed the memoir "a remarkable combination of Oprah's life story and the lessons we can all draw from it for our personal growth."
"We're also thrilled to give a home to Oprah's imprint titles. We all know how extraordinary Oprah's instincts are when it comes to choosing books, instincts borne of her lifelong love of reading and the power of the written word," he added.
Winfrey had published "What I Know for Sure," a collection of her columns from O, the Oprah Magazine in 2014, which became an immediate New York Times best-seller.