The 59-year-old media mogul quickly turned to micro-blogging site Twitter to share her excitement, reported E! online.
"Backstage at Jonathan Ross show London with one direction. Cute boys," Winfrey said.
In the pic, Winfrey poses behind Niall Horan, Louis Tomlinson, Zayn Malik, Liam Payne and Harry Styles, who are all smiles.