The Rs 368.88 crore project, covering 100 km through West and East Jaintia Hills districts, would also affect 121 households through which the NH-44 passes in Meghalaya.
The road project passes through a 10 km stretch through the Narpuh Reserve Forest covering an area of about 2 Ha.
The resettlement and rehabilitation of the displaced people alone would cost the government over Rs 9 crore, officials in the National Highway Authority of India said.
The major settlements en route are Pasyih, Ialong of West Jantia Hills, Wapung, Khliehriat, Ladrymbai, Mynkre, Sonapur, Umkiang and Ratachera in East Jaintia Hills district.