Quoting initial estimates, electoral authorities said about 86 per cent of voters had exercised their franchise during the voting, which largely ended on a peaceful note.
This is the first time in Tamil Nadu that voters have been provided with an option of 'NOTA' (None of the Above) as per the Supreme Court guidelines.
The fourth bypoll in the state since 2011 after AIADMK stormed to power, was necessitated following the death of sitting AIADMK legislator C Perumal early this year.
While AIADMK supremo and Chief Minister J Jayalalithaa went on a day-long campaign trail for her candidate P Saroja, Perumal's widow, DMK's first family made its presence felt with party chief M Karunanidhi's children M K Stalin and Kanimozhi canvassing votes for their party nominee V Maran.
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The result of the elections will not, however, alter the arithmetic in the state assembly as AIADMK enjoyed an absolute majority of 151-members in the 234 member house.
The opposition party's win would, however, send warning signals to the ruling dispensation ahead of next year's Lok Sabha polls.
Counting of votes will be held on December 8.