Mixed doubles defending champions Sania and Bruno Soares lost 3-6, 3-6 to Czech Republic's Andrea Hlavackova-Lukasz Kubot in a first round contest lasting 65 minutes.
Earlier in the day yesterday, Leander Paes and Fernando Verdasco lost their second round match 5-7, 6-4, 3-6 to unseeded American giant-killing pair of Steve Johnson and Sam Querrey.
Paes and his Spanish partner Verdasco got the better of Germans Florian Mayer and Frank Moder 6-2 6-3 in the opening round of the men's doubles competition.