In a chat with media here, he said that Pakistan had taken up the issue of "RAW's activities" with Indian authorities on several occasions.
The foreign secretary said that two persons recently arrested in Karachi confessed to having received training for militancy from the Indian spy agency.
He also talked about the bus attack in Karachi and said if RAW was found involved in the massacre of Ismailis, Pakistan would take up the issue in all international forums.
Last week, Pakistan's army chief had also expressed concerns over activities of RAW in the country.
Reportedly, Pakistan also raised the issue with Afghanistan when Prime Minister Nawaz Sharif visited Kabul this week.