The fishermen were arrested on the Arabian Sea off the coast of Badin district in southern province of Sindh.
An official of Pakistan Maritime Security Agency (PMSA) has confirmed that "27 Indian fishermen were arrested with their four fishing vessels."
He said the fishermen will be handed over to police here to register cases against them.
Maritime officials of India and Pakistan regularly arrest fishermen who stray in to each other's waters due to poorly marked sea boundary.
Pakistan released 163 Indian fishermen in August ahead of a meeting of their national security advisors which was later called off.