The 219 fishermen were freed from Malir jail on instructions from the Interior Ministry as a goodwill gesture, jail superintendent Hasan Sehto said.
This is the second batch of Indian fishermen released from Pakistan jails since relations between the two countries became tense after the terror attack on an Indian army base in Uri in September for which India has blamed Pakistan-based Jaish-e-Mohammed terror outfit.
Sehto said the Indian fishermen who were released today would be handed over to Indian officials at the Wagah border.
He said that around 110 more Indian fishermen remain in Landhi jail in Karachi.
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Last March, the Pakistan government had released 87 Indian fishermen who had been languishing in jail in Karachi for the last two and half years.
Pakistan and India frequently arrest each others' fishermen for violating the territorial boundary.
Last Friday, Pakistan Maritime Security Agency arrested 66 Indian fishermen for illegally fishing in Pakistan's territorial waters.
Fishermen from both countries end up languishing in jails for years even after serving their sentences and their only hope of getting released is when the governments decide to take goodwill steps.