Security forces thwarted the stabbing attempt by a Palestinian armed with a knife at the Beit Einun junction northeast of Hebron, a military statement said.
"Forces on site responded and fired towards the attacker, resulting in his death," it said.
On Tuesday two young Palestinians were shot dead after one of them also tried to stab Israeli soldiers in the same area.
Israel and the Palestinian territories have seen a wave of Palestinian attacks in recent months, with 23 Israelis killed since October 1.
Tensions have been particularly high in Hebron, where several hundred Israeli settlers live under heavy military guard in the heart of the city among about 200,000 Palestinians.