Nearly half of those were seriously hurt yesterday when the run in the northern Spanish town resulted in a bloody human pile-up that got trampled by the half-tonne bulls, sending 23 revellers to hospital.
As on each of the last eight mornings, a firework set off today's mad dash through Pamplona's cobbled streets of six bulls and six steers as well as hundreds of thrill-seekers, many dressed in traditional white with a red neckerchief.
The early morning bull runs are the highlight of the fiesta, which was immortalised in Ernest Hemingway's classic 1926 novel "The Sun Also Rises" and now draws hundreds of thousands of tourists each year.
The regional government said five runners were hospitalised today including a 23-year-old Australian woman with a gore wound.
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During Friday's run, bulls gored three men including a 20-year-old American.
Each year, hundreds of people are treated by medics and the Red Cross at the scene for cuts and scrapes without being hospitalised.