In a message to AIADMK MLAs on the eve of the vote on confidence motion, Panneerselvam said Jayalalithaa had groomed all of them and given an identity.
Unmindful of her health, she had toured the entire state to ensure that "family rule was not established in the state", he said in an apparent reference to the DMK.
Referring to the 2011 expulsion of AIADMK General Secretary V K Sasikala from the party by Jayalalithaa, Panneerselvam asked if the MLAs could aid the "family rule" of such expelled persons.
"Isn't this a betrayal of Amma and the people who had reposed faith in her by voting for her?" he asked.
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Panneerselvam said he had been insisting that a government desired by Jayalalaithaa should continue and expressed joy that among others, Mylapore MLA and former DGP R Nataraj had backed him.
"For the fulfilment of Amma's wishes and those of students, youngsters, women and all the people of Tamil Nadu please vote against (the Chief Minister) in tomorrow's vote of confidence", he said, adding the MLAs should use this "last minute opportunity in the right way".
He urged the family members and the constituency members of the respective MLAs to advise them appropriately on this matter.