"Party is above everyone. No one is bigger (than it)," Roy, still an all India general secretary of TMC, told reporters, in an apparent reference to the party supremo.
On being pointed out that he was with the party from the beginning, he curtly replied "I am still with it".
Banerjee had clipped Roy's wings by appointing party MP Subrata Bakshi the additional all India general secretary in an organisational reshuffle on February 14.
TMC leadership had yesterday accused Subhrangshu of apparently breaching discipline by making certain remarks against the party.
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Subhrangshu, whose role was questioned by the party for the decline in its voteshare at Kalyani Assembly segment under Bongaon Lok Sabha seat in the recent by-election, had said that at a meeting before it, in which all party MPs, MLAs were present, he was not given any responsibility.
The party, he said, must also investigate how it lost in some areas of Assembly segments of Lok Sabha constituencies like Barrackpore, Barasat, Serampore and south Kolkata during the last Lok Sabha polls.
"No one will be spared if any intent of anti-party activity is found," TMC secretary general Partha Chatterjee had stated yesterday while not ruling out disciplinary action by the party against Subhrangshu.