On another day punctuated by heavy showers and thunderstorms, meaning long delays for the players, Czech Pliskova beat Zhang Shuai of China 6-2 6-0, then Dominika Cibulkova in an exciting semi-final 6-4 4-6 6-3.
Her playing day began at 12:30 local time (0930 GMT) ending just before 11pm.
Similarly Dane Wozniacki first beat American qualifier Lauren Davies 7-5 6-1, then Olympic gold medallist Monica Puig 6-1, 6-2, finishing past midnight.
Puig was also playing her second match of the day having earlier completed her victory against Daria Kasatkina of Russia, 6-4, 5-7, 6-4.
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Pliskova, speaking before she knew who her final opponent would be, said "every final is tough".
The pair have met three times before, with Wozniacki winning on every occasion. However they have not met since 2014. Pliskova, 24, is ranked number three in the world, Wozniacki, who is two years older, is number 18.
Incredibly, it also means the rain-ravaged tournament may even finish on time, despite the schedule being battered because of the terrible weather conditions throughout the week.
Friday was one of the worst days yet, with delays up to around five because of thunderstorms. It took some 12 hours to complete just five singles matches.
Tariq Darwish Zainal, secretary general of the Qatar Tennis Federation, said play would not continue after Sunday even if the final matches could not be played.
Rain has delayed play on every single day, virtually wiping out all matches on two separate days.
The Qatar Open is scheduled to finish on Saturday, but has the option of extending to Sunday. Zainal added that Doha is looking at building a new 12,000-seater stadium, which will include a roof. This could be ready by 2021, he said.