"The horse passed away at 5.30 PM. He was suffering from medical-related complications. The exact cause will be known only after post-mortem," IG Garhwal Sanjay Gunjyal said.
Shaktiman's story stirred many across the country after it was seen in television visuals taking multiple blows and dragging its broken, mangled limb in excruciating pain during the protest on March 14.
The 13-year-old didn't respond well to a prosthetic leg brought from the US, doctors said. It spent the past few weeks at the police lines in Dehradun. It was a well trained horse and part of Uttarakhand Mounted Police for years. It had participated in several parades.
BJP MLA Ganesh Joshi, accused of instigating the brutal attack on the horse, said, "I am very sad. I was not at fault, not even 0.1 per cent. If I am found guilty, then cut my leg."
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The lawmaker was arrested and later let off on bail last month.
In a sharp reaction, Union minister Maneka Gandhi said, "Shaktiman was a police officer on duty and the person who put him through so much pain and ultimately killed him should be arrested for killing a police officer."
Expressing shock over the demise of the horse, former Uttarakhand Chief Minister Harish Rawat said,"It was a brave soldier of Uttarakhand and died while performing its duty."
The horse had lost its leg after it was brutally beaten during clashes between BJP protesters and the police on March 14.