The pontiff called on all parties to "spare no effort in urgently ending violence and a conflict which has already caused too much suffering".
"Today, in Montreux in Switzerland, an international conference in support of peace in Syria begins," he told pilgrims gathered for the weekly general audience in St. Peter's Square.
"I hope for a decisive path to reconciliation, understanding and reconstruction for the dear Syrian nation, with the participation of all citizens, where everyone can find in the other, not an enemy, not a rival, but a brother to accept and hug."
"I pray to the Lord that he touch everyone's heart... For the greater good of the Syrian people, who have suffered so much," the pontiff said.
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Erupting after the regime cracked down on protests inspired by the Arab Spring, the civil war has forced millions from their homes.
Recent months have seen the conflict settle into a brutal stalemate- with the death toll rising but neither side making decisive gains.