The short "Motu Proprio", a decree of Francis's own initiative, strengthens the supervision of financial transactions "in response to a recommendation of the Moneyval Committee," the European watchdog which carried out a review of the Vatican bank last year.
It includes the creation of a new Financial Security Committee to help increase vigilance and coordinate efforts in countering corruption.
It also extends laws which previously only applied to the tiny state to the "institutes and entities dependent on the Holy See, as well as to nonprofit organisations" -- such as the Caritas International humanitarian organisation.
"It is a means of ensuring the road (towards transparency) continues," Vatican spokesman Federico Lombardi said in a press conference.
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"In today's world, it is all about resisting increasingly insidious forms of financial criminality. We have to be equal to the challenges in order to protect legality, and not be left behind," he said.
The Vatican is attempting to reform its finances to get onto a "white list" of states that respect international fraud rules.
The 76-year-old pontiff followed this by installing a special five-member commission tasked with investigating the bank and reporting their findings directly back to him personally.
The commission's first report is expected in October, and may spark wider reforms of the murky institute.
A US consultancy firm, Promontory Financial Group, has also been tasked with conducting an external review of the bank's rules on money laundering.