The Yellow Cloud guitar, said to be one of Prince's favourites, was bought for USD 137,500 by the owner of the American Football team Indianapolis Colts.
Bowie's hair, which sold for USD 18,750, came from a former employee of Madame Tussauds in London who had used it to recreate the music icon's 1983 hairdo.
Both stars died earlier this year.
Prince died from an accidental overdose at his Minnesota home in April while Bowie died of cancer in January.