Supporters of Shiromani Akali Dal (Amritsar), led by former MP Dhian Singh Mand, assembled at Golden Temple and raised 'Khalistan Zindabad' slogans besides brandishing swords.
As many as 25 Sikh youths were detained, police said.
Tight security arrangements were made by Punjab police to prevent any untoward incident. Policemen in civil dress along with SGPC task force were present on premises of the Golden Temple, officials said.
Heavy barricading was done by the SGPC officers around Akal Takht to prevent entry of any suspicious element to disturb peace, police said.
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Addressing the gathering, Jathedar Akal Takht said, "Sikh community will, all over the world, remember the unhealed wounds of Operation Bluestar."
Memorial made in the memory of those killed during Operation Bluestar in 1984 witnessed sizable footfall of devotees today.
Radical Sikh group Dal Khalsa has called for Bandh here in the memory of those killed during the Operation in June 1984 in Punjab.
Punjab Police personnel conducted a flag march in Amritsar on June 4 in areas leading to the Golden Temple Complex where the Operation Bluestar was undertaken in 1984 to flush out militants.
Punjab Police sources had said that the security personnel in the districts have been asked to remain extra alert in view of the killing of a Sikh youth during protests in Jammu yesterday against the removal of posters of Sant Jarnail Singh Bhindranwale.