Indrani is in critical condition at J J Hospital where she was admitted yesterday after being taken from Byculla prison in an unconscious state following allegedly taking an overdose of pills.
Late last night, government asked IG (Prisons) Bipin Kumar Singh to inquire into the suspected drug overdose episode.
Principal Home Secretary (Prisons) Vijay Satbir Singh said the probe would cover all aspects, including whether the medicines or any other toxic she consumed were prescribed by doctors and if there was an overdose and how it happened.
The report of Indrani's stomach wash was negative, while the result of blood and urine samples sent to Kalina Forensic Laboratory for testing was expected by tomorrow, Vijay Singh said.
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43-year-old Indrani was on Mirtaz, an anti-depressant, and Etizolan, a sleeping pill, he said.
"After she regains consciousness, the statement she gives will provide vital information on how she fell unconscious and if there was a drug overdose, how did that happen. If there is element of criminality, a case will be filed against those guilty," he said.
Her former driver Shyam Rai and ex-husband Sanjeev Khanna have also been arrested in the sensational case whose probe is being handled by CBI.