Advocate Shrikant Shivade, Salman's lawyer, cross-examined the prosecution witness Dr Shashikant Pawar who was with the government-run J J Hospital here back then.
Khan's car had rammed into a roadside shop in suburban Bandra, killing one person and injuring four people, who were sleeping on the pavement, on the night of September 28, 2002.
He was taken to the J J Hospital after the mishap for medical examination. Pawar told the court today that doctors collected Salman's blood and put it in two vials before sending it to the laboratory.
The defence also pointed out that preservatives should have been added to the sample as per the procedure but it was not done.
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Dr Pawar's cross examination would continue on Saturday.
Meanwhile, special public prosecutor Pradeep Gharat today sought the court's permission to examine three more witnesses whose statements were not a part of the charge sheet.
Twenty-one witnesses have been examined so far, with another five to six left.
Salman is facing the charge of culpable homicide not amounting to murder, which attracts maximum of ten years in jail.