The villagers said the four buffaloes died last night at Mandola village.
Scores of buffaloes had died of a mysterious disease at Zenabad, Dahina and neighbouring villages of the district in the past three weeks, but the Veterinary Department had not yet been able to identify the disease and provide treatment, they claimed.
The road blockade, which lasted for several hours, was withdrawn after the SDO of the Animal Husbandry Department, Nasib Singh, assured the villagers of speedy and free treatment of sick animals.