The protesters alleged that some people also misbehaved with the ambulance driver after stopping the vehicle from ferrying a critical patient to Jammu from Rajouri.
They shouted anti-government slogans and demanded registration of a case against the accused.
SP Rajouri M Haseeb "we are trying to resolve the issue".
Ravinder Raina, who is local MLA and senior BJP leader, said it is an attempt by some vested interests to disturb peace and trigger violence.