The united Andhra Pradesh supporters organised rallies, fasts, human chains, bike rallies, demonstrations and road blockades at various places in the two regions.
Normal life was badly affected due to a 48-hour shut-down called in Krishna district by the Andhra Pradesh Non-Gazetted Officers' Association (APNGOs).
State transport buses were off the roads and several business establishments remained shut in Krishna district, a report from Vijayawada said.
Coastal Andhra and Rayalaseema regions have been witnessing a wave of protests ever since the Congress Working Committee gave its nod to the formation of separate Telangana.
Transport Minister B Satyanarayana, who is also the state Congress president, held talks with the striking employees of Andhra Pradesh State Road Transport Corporation, seeking to end their stir, but the matter remained inconclusive.