Putin has ridden a Harley-Davidson with the bikers and called them his "brothers". He is said to be close to its long-haired leader, Alexander Zaldostanov, nicknamed "the Surgeon".
The patriotic group opposes Ukraine's European integration and the protest movement that has taken power in Kiev. Its website says its members are "ready to die like warriors".
"Tomorrow people are organising an action called Russian Spring," Zaldostanov told the RIA Novosti news agency, saying the event would start from the town of Popasnaya in eastern Ukraine.
The overwhelmingly Russian-speaking peninsula of Crimea has emerged as a flashpoint in Ukraine after the ousting of president Viktor Yanukovych, with many residents openly hostile to the new authorities in Kiev.
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The bikers' leader said members also planned to deliver "humanitarian aid" to Sevastopol on four-wheeler bikes.
Putin has several times appeared at bike rallies with Zaldostanov, a towering figure who wears studded leather and ties his long hair back in a pony tail.
In 2011, Putin, riding a Harley-Davidson, led a column of the bikers at a rally held on a former Soviet warship in the Russian Black Sea port city of Novorossiisk.
The club has its own Kiev branch, which says its aim is to "spread Russian influence around the world".