The re-instated service will take the airline's total weekly passenger flights from 95 to 102 across 13 key cities in India.
Qatar Airways had previously served Nagpur from September 2007 to May 2009.
The decision highlights the importance of its ongoing expansion and commitment to the Indian market.
"Reinstating the Doha-Nagpur route is part of Qatar Airways' strategy to service increasing demand by providing the best possible flying experience to our customers in India.
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Qatar Airways will operate a daily A320 on the route between Doha and Nagpur from December 1, with an average flight time of just over four hours.
A new economic zone and airport are being planned in Nagpur, which are likely to increase the attractiveness of Nagpur and Qatar Airways will be poised to meet the demand in the market, Al Baker said.