A ComRes survey for BBC local radio for Faith in the World Week, which is exploring the healing power of music, offered 1,000 people 10 choices.
'Dancing Queen' by Abba came second, with 'Happy' by Pharrell Williams in joint third with "classical music" generally.
Faith in the World Week, which runs until November 2, is examining how music can change and shape people's lives.
Overall, 81 per cent of Britons say that they have been made to feel better by listening to music. Just 36 per cent said that they prefer to listen to speech than music when they are not feeling well, the poll found.
Among younger adults, 'Happy' was the most popular song. Songs by Bob Marley, Robbie Williams and Michael Jackson were also on the list presented in the survey.
'Bohemian Rhapsody' is taken from Queen's A Night at the Opera album. Nearly six minutes long, the track went to number one in the UK singles charts on two occasions - on its initial release in November 1975, and in 1991 following the death of the band's frontman Freddie Mercury.