The 3D reboot, directed by Zebediah De Soto, is a reimagining of George A Romero's hit black-and-white movie. It revolves around a group of survivors fighting to stay alive when a mysterious plague unleashes the undead on New York City.
Madhavan, 43, will join actors like Danielle Harris, Tom Sizemore, Tony Todd, Alona Tal, Sarah Habel and Bill Moseley.
"It's a great introduction to American Cinema, working with Simon West and his team. He's made and continues to make some of the most exciting and thrilling films out of Hollywood. I am honored to be working alongside him. I am also thrilled to be in this 3D re-imagining of the cult-classic 'Night of The Living Dead'," Madhavan said a statement.
"This movie represents a whole new way of visualizing the classic zombie genre. It has a fresh and exciting style that sets it apart from all other horror films seen up until now," said West, who has produced action hits "Lara Croft: Tomb Raider", "Con Air" and "Black Hawk Down".