"Rules can be framed to regulate the sport to prevent injuries, but instead of that (framing rules), is it right to negate a culture?" he asked.
Stating that the sport was the cultural heritage of Tamil people, he said it "should be protected".
"Jallikattu should be held to uphold the traditions of our Tamil culture," he said.
Participating in a function yesterday, he said while rules could be brought in, the sport should be held in sync with tradition and culture.
Actor Arya has also spoken in favour of the sport.
In the function held by a Tamil magazine, Rajinikanth was awarded the best actor for his performance as a lively don despite his advancing age in last year's Tamil hit movie "Kabali".