The test was conducted as two separate birth certificates of the girl, who claimed that she was 16, were found during investigation.
"The ossification test was conducted to ascertain her exact age. The test was conducted at the Goa Medical College and Hospital," a senior Crime Branch official told PTI.
He said the measure is required as an evidence.
"A panel of three doctors conducted the test and results are yet to be announced," the official said.
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Monserratte, former education minister, was arrested on May 5.
Police had also arrested the girl's mother and another woman, Rosy Ferros, for allegedly selling her to Monserratte for Rs 50 lakh.
The girl, who was working in Monserratte's lifestyle store at Miramar beach in Goa, had claimed that she was raped in March, a charge rejected by the legislator.
Crime Branch is expected to record statement of Monserratte's wife, Jeniffer, who is also an MLA from Taleigao constituency.
On Sunday, the MLA was subjected to psychiatric tests by police.
Police had examined 10 witnesses and also recorded the girl's statement before a magistrate.
The girl had alleged that Monserratte kept her in confinement, administered some stupefying substance and had sexual intercourse with her several times without her consent.