The team found numerous fragments of glass during a three-year dig in the ruined ancient city of Castulo and in July dug up some bigger bits with designs that caught their eye.
When pieced together, the shards formed a plate dating to the fourth century AD, with an engraving of Christ in a Roman-style toga, neatly groomed.
The researchers identified it as a paten, a plate for holding the bread for communion.
It went on display this month in a museum in the southeastern Spanish town of Linares.
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The leader of the dig, Marcelo Castro, hailed the finding as "an exceptional archaeological document".
Three haloed figures are engraved on it, with Christ himself in the middle, holding a cross and a Bible, flanked by two men thought to be the apostles Peter and Paul.
Experts say Christ's lack of beard and short curly hair in the engraving are very rare traits in historic depictions of him.
Previously Christians had worshipped in secret and produced few sacred images for fear of persecution under the Romans.
The Roman style of the paten was "present in the first moments" of Christianity post-Constantine but was "discarded later on in favour of other ways of depicting Christ", Castro told AFP.
The team of archaeologists believe the plate was made in the Ostia glasswork district of ancient Rome.
Other rare examples of similar early Christian art are held in Paris's Louvre Museum and the Toledo Museum of Art in Ohio, he said.