Dhinakaran said the video had not been released so far since Jayalalithaa was in a "nightie".
His remarks came a day after the opposition led by the DMK called for a CBI probe into the treatment and death of the former chief minister, citing a state minister's recent remarks that AIADMK leaders "lied" about Jayalalithaa's health during her hospitalisation.
The video of Jayalalithaa, attired in a "loose nightie" was shot by Sasikala after the former was shifted from the ICU at Apollo Hospitals to a room, he told reporters here.
According to Dhinakaran, when the opposition had earlier raised doubts over Jayalalithaa's treatment and death, Sasikala had herself suggested that "we ourselves should seek a judicial probe" into the matter.
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"So, we are ready to submit it at an appropriate forum, whether CBI or even Interpol. We have nothing to fear," he said.
Over Chief Minister K Palaniswami ordering setting up of an inquiry commission, headed by a retired high court judge, to probe Jayalalithaa's death, Dhinakaran said it was only an attempt to "convince" his now deputy O Panneerselvam, who had earlier raised doubts over the matter.
Dhinakaran said any commission probing the matter should be headed by a "senior sitting judge and not a retired one."
Referring to DMK Working President M K Stalin's appeal for a CBI probe into the former chief minister's treatment and death, Dhinakaran claimed such a demand was being made ever since the "wrong campaign" against Sasikala by them and others was fading.
"The wrong campaign against Chinnamma (Sasikala) does not exist anymore among people and therefore they are making all kinds of attempts," he charged.
The opposition had yesterday called for "unravelling the mystery" behind the death of the AIADMK supremo, who passed away on December 5 last year after 75 days of hospitalisation.
No one was allowed to meet the late chief minister. Whoever came, would be briefed by Sasikala's relatives that she was "all right", he had said.
Yesterday also, Srinivasan said, "Sasikala and her family would tell us she (Jayalalithaa) ate idli, had coffee, was given sweets, did that and this... we told what she (Sasikala) said."
Dhinakaran alleged Srinivasan was making such comments to save his position.
People realised that all such attempts were being made by them to stay in power, he said.
Hitting out at Panneerselvam, Dhinakaran said, "The day he joined hands with Palaniswami, people realised that his six-month old dharma yudh (against Sasikala) was for post only, for the post of deputy chief minister."
Earlier, it was Sasikala who had nominated him as the chief minister following the death of Jayalalithaa, he claimed.