Researchers are now trying to use those and other sounds tigers make to help protect and boost their population in the wild.
The effort, called The Prusten Project, is the idea of Courtney Dunn, who currently works at the Dallas World Aquarium as a senior mammal keeper and has a master's degree in biology. Prusten is another word for chuffing.
"What we have discovered with our research is that tiger voices can be used like a fingerprint for individuals, like a vocal fingerprint as unique as you and I," Dunn said.
Project officials are using those recordings to build a computer program to help identify specific tigers and determine more accurate population numbers so that organisations know where to focus their protection efforts.
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They can also listen in to see if any poaching activity is occurring.
Dunn says they also can distinguish between male and female sounds and knowing that information will tell them if there is a healthy breeding population.
The Milwaukee County Zoo recorded audio from four tigers, before they sent one female off to another zoo to make room for possible offspring.
"Most zookeepers, we get into this because we love animals and we love conservation," said Amanda Ista, zookeeper in the big cats area at the Milwaukee County Zoo. "To be part of a project that is directly linked to conservation is a really cool thing for us."
According to the World Wildlife Fund and Global Tiger Forum, there are nearly 3,900 wild tigers, mostly in Asia, compared with an estimated 3,200 in 2010.
"They are helping their cousins in the wild, and right now tigers need the help in the wild as much as they can get," Ista said.