"A minimum number of five people have been identified from bone remains, four of whom were buried and one of whom was burned," the ministry said in a statement.
"The dead are a woman, two middle-aged men and a newborn" and a fifth person whose sex could not be verified, it said.
The woman was estimated to be aged over 60, and the two men between 35 and 45 years old, officials said. No other details were immediately available on the baby and the fifth person.
The ministry had originally thought that a single skeleton lay in the tomb, but on Monday said that out of 550 bone fragments found, 157 had been matched to specific bodies so far.
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Other bones were those of animals, including a horse.
The tomb, measuring 1,640 feet in circumference and dug into a 30-metre hill -- was found to contain sculptures of sphinxes and caryatids, intricate mosaics and coins featuring the face of Alexander the Great.
The tomb's location was known in antiquity, and it is believed to have been repeatedly looted following the conquest of the ancient Macedonian kingdom by Rome in the second century BC.
Few relics were found, and the culture ministry confirmed today that even the grave inside the tomb had been searched.
"The condition in which the bones were found indicates that they had been disturbed," it said.
In December, the ministry dismissed as "unfounded" claims on Greek websites that the bones were those of a 54-year-old woman, and is therefore likely to be Olympias.
Additional research will determine whether the dead were related, the ministry said.