Renault CEO Carlos Ghosn described the project as a "strategic alliance" that would open up "new frontiers" for the automaker.
"China has the biggest growth potential in the world," he said, adding: "Dongfeng has paramount knowledge of the Chinese market."
There were many opportunities in China, especially in developing electric vehicles, Ghosn said.
"This project is about exploring new frontiers. The challenge is big but we are ready and well-prepared."
The joint venture in Wuhan, in the central province of Hubei, is expected to start production in 2016 and will have an initial capacity of 150,000 vehicles a year, but production is expected to double.
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The plant in Wuhan will begin by assembling urban SUVs, of which Renault already sells tens of thousands of units a year in the Chinese market.
The two groups will also set up a research and development centre in China with 200 staff to develop new technologies for electric and hybrid cars.
Renault was previously the only global top 10 carmaker not to have a manufacturing plant in China.
It already has a partnership alliance with Nissan, and is the Japanese firm's biggest shareholder.
But analysts say Nissan's sales are hit when diplomatic tensions mount between Beijing and Tokyo.
"I hope we're going to see a new success with Dongfeng-Renault," said the Chinese firm's president Xu Ping, adding that it "will help us to go global".
The project with Renault has been a decade in the making, after a previous unsuccessful joint venture by the French firm in China.
Renault began operations with China Space Sanjiang Group in 1993 to manufacture the Traffic minibus, but production stopped in 2003. Dongfeng and Renault have been in talks since then.