Fed officials' highly hawkish comments mainly soured forex market sentiment.
Dealers said dollar flexing muscles overseas and its increased demand from importers put the squeeze on the rupee.
They said, however, a higher opening in the domestic stock market limited the rupee losses.
Yesterday, the rupee had retreated after a three-session winning run and lost 28 paise to end at 64.56 against the dollar with simmering geopolitical tensions taking the centre-stage at the global level.
Meanwhile, the benchmark BSE Sensex recovered 55.50 points, or 0.18 per cent, to 29,631.24 in the beginning.