The demonstrators had gathered near Red Square late yesterday in support of activists jailed earlier in the day for staging "mass riots" in May 2012, a key case seen as a symbol of the harsh crackdown on dissent under President Vladimir Putin's latest term.
Police arrested some 420 demonstrators around the Manezhnaya Square and surrounding streets on various charges, including taking part in an unsanctioned rally and resisting police.
Amnesty International condemned the detentions, saying: "the Russian authorities' rampant violation of freedom of expression and assembly shows no sign of letting up."
The detentions came a day after the closing of the Winter Olympic Games in Russia's southern resort of Sochi and against the backdrop of months of mass, often deadly unrest in neighbouring Ukraine that over the weekend ousted the ex-Soviet country's pro-Kremlin president.
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Among those detained late Monday were the two members of Pussy Riot protest group released from penal colonies last year, Nadezhda Tolokonnikova and Maria Alyokhina.
Protest leader and runner-up in last year's Moscow mayoral race Alexei Navalny was also detained and charged with a more serious offence of disobeying police, which carries a maximum punishment of 15 days in jail.
Opposition politician and former cabinet minister Boris Nemtsov was also charged with disobeying police.
Both were taken to Moscow's Tverskoi's district court today after the spending the night in police custody.
The protesters were demonstrating against the sentencing of eight activists for participating in a May 2012 rally on the eve of Putin's inauguration for a third presidential term that ended in scuffles with police.
The activists were convicted of "mass riots" and using physical violence against police, charges that were condemned as excessive by their lawyers and supporters.
One activist, 22-year-old Yaroslav Belousov, said that he simply threw a lemon at police ranks as tens of thousands marched in protest against Putin's return to the presidency.
Most of the activists have been held in custody since 2012, meaning that several will be eligible for parole later this year.