The Russian warplanes hit IS command centres, weapon caches, artillery and communication posts in the Homs, Idlib and Latakia provinces of Syria, the ministry said in a statement.
"25 sorties were completed by Su-34, Su-24 and Su-25 jets from the airbase in Hmeimim. Nine IS facilities were struck," the statement said.
In the northwestern Idlib province the Russian military said it had destroyed a training camp by the city of Jisr al- Shughur, artillery positions near Jabal al-Qobeh and armoured vehicles.
Russia last Wednesday launched airstrikes in Syria and says it is hitting targets connected to IS jihadists in the war-torn country.
The United States and its allies have accused Russia of seeking to buttress its long-standing ally President Bashar al-Assad under cover of a claimed assault on IS.