"Currently talks are suspended," Energy Minister Alexander Novak said, quoted by RIA Novosti state news agency.
"Work on formulating agreements on TurkStream is suspended," particularly because "an intergovernmental commission on trade and economic cooperation has stopped meeting" under Russia's retaliatory measures against Ankara, Novak said.
The TurkStream project would see four pipelines carrying Russian natural gas under the Black Sea, linking southern Russia to western Turkey.
Russian President Vladimir Putin announced the plan for the Turkstream pipeline in December 2014, after Russia junked its South Stream joint venture with EU firms, which would have taken gas to southern Europe and Bulgaria.
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The head of Russia's state gas giant Gazprom, Alexei Miller, told journalists earlier today that Turkey would have to ask Russia to renew talks on TurkStream.
"If Turkey considers it needs this project, it can contact us," RIA Novosti quoted Miller as saying.
Gazprom in early October said that the pipeline project would be delayed as tensions heightened after Russia launched airstrikes against Islamic State and other targets in Syria, with its planes several times violating Turkish airspace.