Consumer safety agency Rospotrebnadzor said it had temporarily closed a McDonald's restaurant in the southern city of Stavropol, citing unspecified sanitary violations.
Russia last week shut three McDonald's in Moscow and launched inspections of the company's more than 400 restaurants across the country.
McDonald's Russian unit was not immediately available for comment on Monday evening.
While ostensibly for health reasons, the move against the US burger chain came close on the heels of the West ratcheting up sanctions on Moscow for its role in the Ukraine crisis -- and Russia banning most US and EU food imports in retaliation.
While McDonald's is a symbol of American culture, the company has made huge efforts to build up its local supply chain and says it buys 85 percent of the food it serves in Russia inside the country.