Navalny's video showed a lavish villa purportedly owned by a son of Putin's friend that the president had allegedly used.
The online video accusing Putin of corruption has been seen at least 2 million times since its release yesterday.
The video included aerial footage of the seaside mansion surrounded by a large park near Vyborg, close to St. Petersburg.
It's the latest in a series of YouTube videos by Navalny exposing alleged ill-gotten assets of senior government officials and Kremlin-friendly tycoons.
Navalny, who rose to prominence with his corruption investigations, has declared his intention to run for president in the March 2018 election.