The Syrian Observatory for Human Rights, based in Britain, said nine children were among those killed as they tried to cross the river aboard rafts, escaping from areas where Russian-backed regime forces are battling the Islamic State group.
The monitor, which earlier gave a toll of 20 in the strikes, said the dead included an Iraqi family.
Deir Ezzor borders Iraq and civilians have fled into the province to escape the battles against IS in their own country.
The second is being fought by the US-backed Syrian Democratic Forces, an alliance of Kurdish and Arab fighters, on the eastern bank of the river.
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The Observatory relies on a network of sources inside Syria, and says it determines whose planes carry out raids according to type, location, flight patterns and munitions used.
The group has reported hundreds of civilians killed in operations against IS in Deir Ezzor and neighbouring Raqa province, where the SDF is fighting with US support to capture the former jihadist bastion Raqa city.
The coalition says it takes all measures possible to avoid civilian casualties and that it investigates each credible allegation.
Last month, it acknowledged the deaths of over 700 civilians in its strikes in Syria and Iraq since 2014.
Russia has not acknowledged any civilian deaths from its strikes since its intervened in Syria's war in 2015, and dismisses the Observatory's reporting as biased.
The deaths in Deir Ezzor today prompted outrage from the opposition Syrian National Coalition which described the incident as a "heinous crime".