"The footage shows elephant calves and juvenile elephants being chained, roped and stretched, shocked with electric cattle prods and hit with bull hooks," the Society for the Prevention of Cruelty to Animals (SPCA) said.
This was done to break the animals' spirit so that they would obey humans, it said in a statement.
"The elephants show signs of crippling injuries with severely swollen legs and feet, debilitating abscesses and wounds," National Council of SPCAs inspector Wendy Willson said.
"The calculated and premeditated cruelty of this nature that took place at this facility is a far cry from the loving sanctuary image that Elephants of Eden/Knysna Elephant Park like to portray," Willson said.
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The SPCA said it laid cruelty charges with the police against Elephants of Eden, the Knysna Elephant Park, their directors and management.
If the case is brought to court and the directors and managers are convicted, they could face sentences of up to three years in jail on each charge and lose all their elephants, Willson told AFP.
"In order to dominate or force one's will onto an animal such as the elephant, force needs to be applied and thus is a recipe for abuse.
"The captive elephant interaction industry is a form of tourism driven by greed and without any conservation benefit," she said.
A growing number of people in South Africa and around the world had been injured or killed as a result of the rebellion of trained elephants kept in captivity, the statement said.