The actor's blood sample was taken to ascertain whether he was driving under the influence of alcohol.
Justice A R Joshi is hearing the appeal filed by Salman against the five-year sentence awarded to him by the sessions court after finding him guilty of ramming his car into a shop in suburban Bandra and killing one person and injuring four on September 28, 2002.
"The tampering (with blood samples) has started right from here," Desai contended. He had argued earlier that the samples, which were sent to laboratory, were possibly not Salman's but somebody else's or they were tampered with.
According to the prosecution, blood test proved that Salman was drunk that night.
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Desai argued that the prosecution did not establish whether disposable sterilised syringes were used and whether a syringe was used immediately (after undergoing boiling for sterilisation) or after some time. "This results in tampering," he alleged.
Desai also reiterated his argument that Salman's written consent was not taken while drawing the blood. "When the blood was drawn, proper procedures and protocols laid down in the law has to be followed," he said.
After the blood is drawn, anti-coagulant and preservative has to be added and it was not known whether the blood sample was shaken properly to dissolve the anti-coagulant, he said.
Arguments would continue tomorrow.