The bandh call was given by Gir Nature Club and supported by various organisations - including trade unions, Sasan Hotel association and guide association.
A rally was today taken out which submitted a memorandum to the Sasan Mamlatdar in this regard, Sasan Hotel Association secretary Pyaru Vadsariya said.
Sasan is a village on the boundary of Gir sanctuary and is the main entry point to it.
"We respect our judicial system but the decision on shifting of lions to Madhya Pradesh has been taken without proper scrutiny," the memorandum said.
"Trans-location of some Asiatic lions to Madhya Pradesh is improper because in the past some lions were shifted there and could not match the atmosphere there and failed to survive," it said adding that "Gir is the only place where the atmosphere is suitable to the Asiatic lions."